Innovative Techniques for Extracellular Vesicle Research at Your Fingertips

VetCore in cooperation with Kau-Strebinger Lab brings together techniques for EV research in a shared facility.

• Instruction and training
• Technical questions
• Experimental planing (and conduction)
• Assistance in data analysis

General information

• Use of devices is subject to mandatory instruction and training.
• Booking of devices via VetCore booking calendar is necessary.
• Facility located at GA06G38 (Histology) - chip unlocking required.
• Special consumables must be brought along.

Lab Equipment

Eppendorf CP90NX Ultracentrifuge with two swing-out rotors, P32ST (40 mL max. nominal capacity; max. 180.000 x g; K-factor: 198) and P40ST (13 mL max. nominal capacity; max. 284.000 x g; K-factor 139). Adaptors for P40ST to centrifuge 0.9 mL tubes available.

Ultracentrifugation is used for high-speed separation of nanoparticles, cell organelles and macromolecules. A major advantage of the ultracentrifuge on site is that you no longer must adjust your samples + tubes to the nearest microgram. Equal volume loading is sufficient, as the centrifuge has an in-build auto-taring system, even at high-speed. An ideal solution for fast and sterile sample preparation.

Particle Metrix ZetaView TWIN x20 Nanoparticle Tracking Analysis (NTA) offering you a state-of-the-art method for quantifying particle concentration, hydrodynamic diameter (size), zeta potential, and fluorescence (ex. lasers: 488, 640).

This technique is used to characterize nanoparticles in suspension in the size range of 10–2000 nm. The NTA setup allows real-time visualization of particles below the diffraction limit of conventional microscopes, in both scatter and fluorescence mode.

Unchained Labs ExoViewR200 uses Single Particle Interferometric Reflectance Imaging Sensing (SP-IRIS), an optical sensing technology that combines interferometry with imaging to detect and characterize individual nanoparticles down to 50 nm, including EVs and viruses (lentiviruses, etc.). Our device's LED wavelengths/ excitation range/ emission range = blue (470/ 465–495/ 505–530 nm), green (567/ 543–568/ 580–608 nm), red (623/ 625–655/ 665–725 nm). The violet laser (415 / 415–425/ xxx nm) is reserved for scatter measurements.

After a customizable immunocapturing step you can size and quantify small EVs and perform surface (and cargo) biomolecular colocalization analysis at single EV level. Capturing and characterization of EVs directly from tiny volumes of cell culture supernatant and biofluids without need for prior EV enrichment is possible. ​