The LSM 880 Airyscan is a confocal laser scanning microscope from Zeiss on an inverted Observer Z1 stand. All components are controlled by the ZEN 2 (black) software. It is able to run multidimensional experiments including multichannel acquisition, z-stacks, tile scanning, and time-lapse. Since it is an inverted microscope, it is suitable for imaging both live cells and fixed samples. It is equipped with six laser lines, detectors for detecting both fluorescence signals and transmitted light DIC, and a z-piezo.

Objectives:
10x/0.45 Plan-Apochr.
20x/0.8 Plan-Apochromat
40x/1.40 Plan-Apochr., Oil, DIC III
40x/1.2 LD LCI Plan-Apochromat autocorr., Silicone
63x/1.40 Plan-Apochr., Oil, DIC III

Illumination:
Halogen lamp HAL 100
Illuminator HXP 120 V

Filters:
#49 | Ex 365 | Em 445/50
#38 | Ex 470/40 | Em 525/50
#43 | Ex 550/25 | Em 605/70

Laser Lines:
Laser Diode | 405
Argon | 458, 488, 514
DPSS | 561
HeNe | 633

Laser Detectors:
Ch1 (PMT) | 371 - 740
ChS1 (GaAsP) | 409 - 695
Ch2 (PMT) | 379 - 759
T-PMT | Transmitted Light
ChA | Airyscan

Airyscan
beam splitter:
458
458/514
458/561
488
488/561
488/561/633

Airyscan
emission filter:
BP 420-480 | BP 495-550
BP 420-480 | BP 495-620
BP 420-480 | LP 605
BP 465-505 | LP 525
BP 495-550 | LP 570
BP 570-620 | LP 645

Live cell imaging:
Incubator XL dark
TempModul S1
CO2-Modul S1

The microscope is accessible to authorized persons only. Instrument training is provided upon request and is provided by core facility personnel.

General conditions of equipment usage:

• For operation, it is mandatory to book the Zeiss CLSM880 in the booking calendar.
• The instrument can be operated only by a single user at a time.
• After the experiment, all slides must be removed from the instrument. If used, oil objectives have to be cleaned with lens paper.
• Users must remove their data from the slidescanner storage within 3 months after the imaging experiment.

Lab rules:

• Access to the microscopy lab is possible with a keycard, which is granted after completed training on one of the microscopes.
• General S1 lab rules apply.

Further details on laboratory rules and instrument usage will be provided in the training sessions.

The software Zen lite for viewing the images can be downloaded from the manufacturer's homepage.