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Axio Imager Z2 – upright widefield microscope (Zeiss)

Location 
GA06G49, VetCore microscopy room I

Contact
Stefan Kummer

Booking calendar
https://vetcore-booking.vetmeduni.ac.at

Further information
BMBWF Forschunginfrastruktur

 

The AxioImager Z2 is an upright microscope from Zeiss allowing brightfield, darkfield, DIC, and fluorescence microscopy. Except the stage the system is fully motorized, and all components are controlled by the ZEN 2012 (blue) software. It is able to run multidimensional experiments including multichannel acquisition, z-stacks, and time-lapse. Since it is an upright microscope, it is suitable for imaging fixed samples.

Objectives:
5x/0.16 Plan-Neofluar
10x/0.3 Plan-Neofluar
20x/0.8 Plan-Apochr.
40x/0.95 Plan-Apochr.
40x/1.3 Plan-Apochr., Oil, DIC III
63x/1.4 Plan-Apochr., Oil, DIC III
100x/1.3 EC Plan-Neofluar, Oil

Lights source:
Halogen lamp HAL 100
Illuminator HXP 120 V

Fluorescence filters:
#49 | Ex 365 | Em 445/50
#34 | Ex 390/22 | Em 460/50
#38 | Ex 470/40 | Em 525/50
#00 | Ex 530-585 | Em LP 615
#43 | Ex 550/25 | Em 605/70
#45 | Ex 560/40 | Em 630/75
#50 | Ex 640/30 | Em 690/50

Camera:
AxioCam MRc5 (color cam)
AxioCam MR R3 (b&w cam)

The microscope is accessible to authorized persons only. Instrument training is provided upon request and is provided by core facility personnel.

General conditions of equipment usage:

  • For operation, it is mandatory to book the Zeiss Imager Z2 in the booking calendar.
  • The instrument can be operated only by a single user at a time.
  • After the experiment, all slides must be removed from the instrument. If used, oil objectives have to be cleaned with lens paper.
  • Users must remove their data from the slidescanner storage within 3 months after the imaging experiment.

Lab rules:

  • Access to the microscopy lab is possible with a keycard, which is granted after completed training on one of the microscopes.
  • General S1 lab rules apply.

Further details on laboratory rules and instrument usage will be provided in the training sessions.

The software Zen lite for viewing the images can be downloaded from the manufacturer's homepage.